The terminal enzymes of cholesterol synthesis, DHCR24 and DHCR7, interact physically and functionally[S]

The terminal enzymes of cholesterol synthesis, DHCR24 and DHCR7, interact physically and functionally[S]

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Cholesterol is essential to human health, and its levels are tightly regulated by a balance of synthesis, uptake, and efflux.Cholesterol synthesis requires the actions of more than twenty enzymes to Ball - Senior Tops - Jacket reach the final product, through two alternate pathways.Here we describe a physical and functional interaction between the two terminal enzymes.24-Dehydrocholesterol reductase (DHCR24) and 7-dehydrocholesterol reductase (DHCR7) coimmunoprecipitate, and when the DHCR24 gene is knocked down by siRNA, DHCR7 activity is also ablated.

Conversely, overexpression of DHCR24 enhances DHCR7 activity, but only when a functional form of DHCR24 is used.DHCR7 is important for both cholesterol and vitamin D synthesis, and we have identified a novel layer of regulation, whereby its activity is controlled by DHCR24.This suggests the existence of a cholesterol #x201C;metabolon#x201D;, where enzymes from the same metabolic pathway Fuel Sensors interact with each other to provide a substrate channeling benefit.We predict that other enzymes in cholesterol synthesis may similarly interact, and this should be explored in future studies.